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Early Infant Diagnosis of HIV Implementation Guide Book 3: Guide for Laboratorians

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English: Web Page


This manual is a training and reference guide for laboratorians providing early infant diagnostic services. As infants retain maternal antibodies to HIV for many months after birth, a diagnostic test based on viral material is necessary for definitive diagnosis of infant HIV infection until 18 months of age. The recommended viral method for early infant diagnosis programs is PCR (Polymerase Chain Reaction) amplification of HIV-1 DNA from peripheral blood mononuclear cells from Dried Blood Spots (DBS). Specific considerations for PCR services are addressed in this guide including: necessary laboratory infrastructure, equipment, supplies, standard operating procedures, quality assurance measures, and appropriate reporting of test results.

Related Resources

Publisher: CDC Global AIDS Program
Language: English
Format: Web Page
Region: Global
Audiences: Laboratory Workers, Program Managers
Topic Area: Testing & Counseling
Resource Type: Program Implementation