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Guide to Implementation of Services for Early Diagnosis of HIV in Infants in Resource-Limited Settings

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Book 3. Guide for Laboratorians

This manual is a training and reference guide for laboratorians providing early infant diagnostic services. As infants retain maternal antibodies to HIV for many months after birth, a diagnostic test based on viral material is necessary for definitive diagnosis of infant HIV infection until 18 months of age. The recommended viral method for early infant diagnosis programs is PCR (polymerase chain reaction) amplification of HIV-1 DNA from peripheral blood mononuclear cells from dried blood spots (DBS). Specific considerations for PCR services are addressed in this guide, including: necessary laboratory infrastructure, equipment, supplies, standard operating procedures, quality assurance measures, and appropriate reporting of test results.

Book 3. Guide for Laboratorians

Manual and Overview of PCR PDF
MS Word
PPT
Cover 345K - -
Introduction to Early Infant Diagnosis Implementation Guide for Laboratorians 366K 36K -
Considerations for setting up a PCR laboratory - 61K -
Standard operating procedures (SOPs) for amplicore DBS PCR - 185K 8.2MB
Sample PCR lab requisition form 46K - -
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Ordering Supplies Excel
PDF
MS Word
Equipment, consumables, and staffing estimates 107K - -
Roche consumable supply boxes for clinics and laboratories - - 29K
SCMS catalog numbers for EID equipment and consumables 51K - -
DBS Card Suppliers - - 31K
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Creating, Packaging and Shipping Dried Blood Spots (DBS) PDF
MS Word
PPT
DBS collection poster job aid - - 7MB
DBS acceptance and rejection criteria job aid 328K - -
DBS packaging poster job aid - - 290K
DBS shipping guidelines - 31K -
DBS storing poster job aid - - 9MB
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Training and Quality Assurance MS Word
Training - African Center for Integrated Laboratory Training 35MB
EQA Proficiency Testing Program 50K
EQA Retesting Program 51K
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Appendices PDF
MS Word
Appendix 1 - Maputo Declaration - 706K
Appendix 2 - Becton, Dickinson and Company Fact Sheet 166K -
Appendix 3 - Becton, Dickinson and Company Press Release, 2007 - 166K
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